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Abstract Near-Earth asteroids (NEAs) are a key test bed for investigations into planet formation, asteroid dynamics, and planetary defense initiatives. These studies rely on understanding NEA sizes, albedo distributions, and regolith properties. Simple thermal models are a commonly used method for determining these properties; however, they have inherent limitations owing to the simplifying assumptions they make about asteroid shapes and properties. With the recent collapse of the Arecibo Telescope and a decrease of direct size measurements, as well as future facilities such as LSST and NEO Surveyor coming online soon, these models will play an increasingly important role in our knowledge of the NEA population. Therefore, it is key to understand the limits of these models. In this work we constrain the limitations of simple thermal models by comparing model results to more complex thermophysical models, radar data, and other existing analyses. Furthermore, we present a method for placing tighter constraints on inferred NEA properties using simple thermal models. These comparisons and constraints are explored using the NEA (285263) 1998 QE2 as a case study. We analyze QE2 with a simple thermal model and data from both the NASA IRTF SpeX instrument and NEOWISE mission. We determine an albedo between 0.05 and 0.10 and thermal inertia between 0 and 425J m −2 s −1/2 K −1 . We find that overall the simple thermal model is able to well constrain the properties of QE2; however, we find that model uncertainties can be influenced by topography, viewing geometry, and the wavelength range of data used. 

Abstract Retrons are bacterial retroelements that produce single-stranded, reverse-transcribed DNA (RT-DNA) that is a critical part of a newly discovered phage defense system. Short retron RT-DNAs are produced from larger, structured RNAs via a unique 2′-5′ initiation and a mechanism for precise termination that is not yet understood. Interestingly, retron reverse transcriptases (RTs) typically lack an RNase H domain and, therefore, depend on endogenous RNase H1 to remove RNA templates from RT-DNA. We find evidence for an expanded role of RNase H1 in the mechanism of RT-DNA termination, beyond the mere removal of RNA from RT-DNA:RNA hybrids. We show that endogenous RNase H1 determines the termination point of the retron RT-DNA, with differing effects across retron subtypes, and that these effects can be recapitulated using a reduced, in vitro system. We exclude mechanisms of termination that rely on steric effects of RNase H1 or RNA secondary structure and, instead, propose a model in which the tertiary structure of the single-stranded RT-DNA and remaining RNA template results in termination. Finally, we show that this mechanism affects cellular function, as retron-based phage defense is weaker in the absence of RNase H1.